Pistachio Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
PV1-EK-96    96 tests
PV1-EK-48    48 tests

Total Assay time
30 Minutes


Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.12 ppm

Range of Quantification:
1 to 40 ppm

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Pistachio Nut Allergen ELISA kit

The allergenic proteins associated with Pistachios have only recently become understood. BioFront’s MonoTrace ELISA kit is highly specific to Pistachio, unlike other methods, which are prone to cross-react to Cashew allergen.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Pine Nut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Pistachio allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to pistachio (Pistacia vera). Pecan (0.001%) was minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other matrices including tree nuts, legumes, seeds, flour, meats and spices) tested using the MonoTrace Pecan ELISA kit


The MonoTrace Pistachio ELISA kit is suitable for the qualitative or quantitative detection of pistachio within
various matrices including cookie, cake, chocolate (including milk, dark and white chocolate) as well as ice cream and flours. Chocolate and ice cream extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, pistachio protein was efficiently extracted and detected from diverse food matrices.

Other matrices may also be suitable for the MonoTrace Pistachio ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a pistachio-spiked sample is recommended to ensure that pistachio protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Pistachio Nut Allergen ELISA kit