Pine Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
PN1-EK-96    96 tests
PN1-EK-48    48 tests

Total Assay time
30 Minutes

Pine nut

Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.24 ppm
Range of Quantification:
2 to 80 ppm

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Pine Nut Allergen ELISA kit

Like Brazil nut and Coconut, Pine nuts (Pignolias) are technically seeds, but are typically classified as tree nuts. BioFront’s MonoTrace ELISA kit is one of the few methods available to accurately detect Pine nuts in foods and surfaces.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Pine Nut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Pine Nut allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to pine nut (Pinus pinea). At concentrations as high as 100,000 ppm Pecan (0.0020%), walnut (0.0019%), and buckwheat (0.0012%) were minimally cross-reactive. No cross reactivity was observed within other matrices tested including a large panel of tree nuts, legumes, seeds, flours,meats and spices.


The MonoTrace Pine nut ELISA kit is suitable for the qualitative or quantitative detection of pine nut within cake, cookies, chocolate and ice cream. Chocolate and ice cream extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, pine nut was efficiently extracted and detected using the MonoTrace Pine nut ELISA from diverse food matrices.

Other matrices may also be suitable for the MonoTrace Pine nut ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix of interest has been validated (cross reactivity & recovery). An internal validation using a pine nut-spiked sample is recommended to ensure that pine nut protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Pine Nut Allergen ELISA kit