Pecan Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
PC4-EK-96    96 tests
PC4-EK-48    48 tests

Total Assay time
30 Minutes


Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.17 ppm

Range of Quantification:
1 to 40 ppm

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Pecan Nut Allergen ELISA kit

Pecans are grown extensively in the Southern US, and BioFront’s MonoTrace ELISA kit is recognized as the best-in-class detection method for Pecan detection. Allergic reactions to Pecan typically last a lifetime, so its accurate detection in foods is of the utmost importance.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Pecan ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Pecan allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to pecan (Carya illinoinensis) and to a lesser extent English walnut (17.8-21.2%). Adzuki bean (0.0002%) was minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other matricesincluding tree nuts, legumes, seeds, flour, meats and spices) tested using the MonoTrace Pecan ELISA kit.


The MonoTrace Pecan ELISA kit is suitable for the qualitative or quantitative detection of pecan within cake, cookies, chocolate and ice cream. Chocolate and ice cream extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, pecan was efficiently extracted and detected using the MonoTrace Pecan ELISA from diverse food matrices.

Other matrices may also be suitable for the MonoTrace Pecan ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix of interest has been validated (cross reactivity & recovery). An internal validation using a pecan-spiked sample is recommended to ensure that pecan protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Pecan Nut Allergen ELISA kit