Macadamia Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
MN1-EK-96    96 tests
MN1-EK-48    48 tests

Total Assay time
30 Minutes

Macadamia nut

Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.13 ppm

Range of Quantification:
2 to 80 ppm

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Macadamia Nut Allergen ELISA kit

Macadamia nuts are seeds, but are classified as tree nuts for allergen purposes. Allergic individuals have shown cross-reactivity to Hazelnuts, but BioFront’s MonoTrace ELISA demonstrates little cross-reactivity between the two.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Macadamia Nut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

Target a highly stable Macadamia allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to macadamia nut (Macadamia integrifolia). When other tree nuts were tested at concentrations as high as 100,000 ppm, only pecan (0.0036%) demonstrated minimal cross-reactivity in the assay while various seeds, meats, and spices showed no cross-reactivity. Other matrices including certain legumes and flours demonstrated minimal cross-reactivity when tested using the MonoTrace Macadamia ELISA Kit.


The MonoTrace Macadamia ELISA kit is suitable for the qualitative or quantitative detection of macadamia
within cookie, cake, milk chocolate as well as ice cream. Chocolate and ice cream extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, macadamia protein was efficiently extracted and detected from diverse food matrices.

Other matrices may also be suitable for the MonoTrace Macadamia ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a macadamia-spiked sample is recommended to ensure that macadamia protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Macadamia Nut Allergen ELISA kit