Hazelnut Allergen ELISA kitrequest-ifu

Description
Competitive Enzyme immunoassay

Product Code
HC9-EK-96    96 tests
HC9-EK-48    48 tests

Total Assay time
30 Minutes

Samples
Hazelnut

Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.04 ppm

Range of Quantification:
1 to 40 ppm

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Hazelnut Allergen ELISA kit

Allergies to Hazelnut may be due to the seed, tree pollen or the nut itself. BioFront’s MonoTrace ELISA kit has been carefully designed to provide optimum detection of Hazelnut in a variety of foods and beverages.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Hazelnut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Hazelnut allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

The assay exhibits strong reactivity to hazelnut (Corylus Americana) and to a lesser extent walnut (1.08%). Pecan (0.011%) and fava bean (0.0018%) were minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other matrices (including tree nuts, legumes, seeds, flour, meats and spices) tested using the MonoTrace Hazelnut ELISA kit.

RECOVERY

The MonoTrace Hazelnut ELISA kit is suitable for the qualitative or quantitative detection of hazelnut within cake, cookies, chocolate, ice cream and spices. Ice cream and ice chocolate extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is required for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, hazelnut was efficiently extracted and detected using the MonoTrace Hazelnut ELISA from diverse food matrices and spices at risk of adulteration.

Other matrices may also be suitable for the MonoTrace Hazelnut ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a hazelnut-spiked sample is recommended to ensure that hazelnut protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Hazelnut Allergen ELISA kit