Coconut Allergen ELISA kitrequest-ifu

Description
Competitive Enzyme immunoassay

Product Code
CN1-EK-96    96 tests
CN1-EK-48    48 tests

Total Assay time
30 Minutes

Samples
Coconut

Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.13 ppm

Range of Quantification:
1 to 40 ppm

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Coconut Allergen ELISA kit

Coconut is botanically considered a fruit but is often classified as a tree nut. Consequently, there is little allergic cross-reactivity between coconut and true tree nuts. Coconut-derived ingredients are increasingly being used in a variety of foods and beverages making BioFront’s MonoTrace ELISA kit a critical food safety diagnostic tool. It is also one of few rapid diagnostic methods in use to detect Coconut, and the most sensitive quantitative test available to the food industry.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Coconut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Coconut allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

The assay exhibits strong reactivity to coconut (Cocos nucifera). At concentrations as high as 100,000 ppm, the assay shows no cross-reactivity to other tree nuts. When assaying legumes, seeds, flours, and spices minimal cross-reactivity was observed to mustard seed (0.0011%), sesame seed (0.0014%), sweet rice (0.0015%) and white rice (0.0014%).

RECOVERY

The MonoTrace Coconut ELISA kit is suitable for the qualitative or quantitative detection of coconut within
various food matrices, including cakes, cookies, chocolates, ice cream, yogurt, milk, and spice products. Chocolate, ice cream, and spice extractions require the addition of 5% (w/v) nonfat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, coconut was efficiently extracted and detected using the MonoTrace Coconut ELISA from common food and spice matrices.

Other matrices may also be suitable for the MonoTrace Coconut ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a coconut-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Coconut Allergen ELISA kit