Cashew Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
CA2-EK-96    96 tests
CA2-EK-48    48 tests

Total Assay time
30 Minutes


Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.12 ppm

Range of Quantification:
1 to 40 ppm

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Cashew Nut Allergen ELISA kit

Cashews are sourced from Africa, Asia and South America. In the US, Cashew nut allergy is the second most commonly reported Tree nut allergy. Unlike other ELISA methods, BioFront’s MonoTrace ELISA kit is highly specific to Cashew.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergencontamination in various food samples and environmental surfaces. The MonoTrace Cashew ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target intact Cashew allergen even post food processing effects
Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to cashew (Anacardium occidentale). At concentrations as high as 100,000 ppmwalnut (0.1297%), pecan (0.0517%), and pistachio (0.0101%) were minimally cross-reactive in the assay.No cross-reactivity was observed in the other matrices including a broad range of tree nuts, legumes, seeds, flour,meats and spices.


The MonoTrace Cashew ELISA kit is suitable for the qualitative or quantitative detection of cashew within cake, cookies, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, cashew protein was efficiently extracted and detected using the MonoTrace Cashew ELISA from common food matrices and spices.

Other matrices may also be suitable for the MonoTrace Cashew ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a cashew-spiked sample is recommended to ensure that cashew is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Cashew Nut Allergen ELISA kit