Brazil Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
BN-EK-96    96 tests
BN-EK-48    48 tests

Total Assay time
30 Minutes

Brazil Nut

Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.14 ppm

Range of Quantification:
1 to 40 ppm

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Brazil Nut Allergen ELISA kit

Brazil nut is the seed of the South American Bertholletia excelsa tree. There are limited methods of detection available which make’s BioFront’s MonoTrace ELISA kit a highly sought after method for the detection of Brazil nut.


The MonoTrace Brazil nut ELISA exhibits strong reactivity to Brazil nut (Bertholletia excelsa). At concentrations as high as 100,000 ppm, the Monotrace Brazil Nut ELISA shows no crossreactivity to other matrices including tree nuts, legumes, seeds, flour, meats and spices.


The Brazil nut ELISA kit (BN-EK) is suitable for the qualitative or quantitative detection of Brazil nut within
various matrices, including chocolate, ice cream, cookie, cereal and spice products. Chocolate, ice cream and spice extractions required the addition of 5% (w/v) non-fat dry milk to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, Brazil nut was efficiently extracted and detected using the Brazil nut ELISA from diverse food matrices and spices at risk of adulteration.

Other matrices may also be suitable. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). An internal validation using a Brazil nut-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Brazil NutAllergen ELISA kit