Almond Nut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
AP1-EK-96    96 tests
AP1-EK-48    48 tests

Total Assay time
30 Minutes


Incubation Times:
10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Limit of Detection:
0.15 ppm

Range of Quantification:
1 to 40 ppm

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Almond Nut Allergen ELISA kit

Almonds are consumed more than any other tree nut and is the tree nut mostly likely to be identified in food allergen recalls. BioFront’s MonoTrace ELISA kit is best-in-class for Almond detection on foods, beverages and surfaces.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Almond ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Almond allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to almond (Prunus dulcis) and to a lesser extent mahalab (0.0546%), a spiceprepared from the pit of cherry (Prunus avium). Cashew (0.0316%), sunflower seed (0.0065%), and oat (0.0012%)were minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed inthe other matrices (including tree nuts, legumes, seeds, flour, meats and spices) tested.


The MonoTrace Almond ELISA kit is suitable for the qualitative or quantitative detection of almond within cake, cookies, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions required the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In summary, almond was efficiently extracted and detected using the MonoTrace Almond ELISA from diverse food matrices and spices at risk of adulteration.

Other matrices may also be suitable for use with the MonoTrace Almond ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using an almond-spiked sample is recommended to ensure that almond is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Almond Nut Allergen ELISA kit