Soy Allergen ELISA kitrequest-ifu

Description
Competitive Enzyme immunoassay

Product Code
SG6-EK-96    96 tests
SG6-EK-48    48 tests

Total Assay time
30 minutes

Samples
Soy

Incubation Times:
10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Limit of Detection:
0.16 ppm

Range of Quantification:
1 to 40 ppm

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Soy Allergen ELISA kit

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Soy ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Soy allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

The assay exhibits strong reactivity to soy (Glycine max). At concentrations as high as 100,000 ppm Chick pea/garbanzo bean (0.0060%), garfava flour (0.0025%), peanut (0.0017%) and lentils (0.0010%) were minimally cross-reactive. No cross reactivity was observed within other matrices tested including a large panel of tree nuts,legumes, seeds, flours, meats and spices.

RECOVERY

Recovery of spiked soy samples at various concentrations was efficient (>79%) when the following food matrices were assayed: chocolate, cereals, breads, and cake.

Soy Allergen ELISA kit