Shellfish Allergen ELISA kitrequest-ifu

Description
Competitive Enzyme immunoassay

Product Code
CR1-EK-96    96 tests
CR1-EK-48    48 tests

Total Assay time
30 minutes

Samples
Shellfish

Incubation Times:
10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Limit of Detection:
0.07 ppm (shellfish)

Range of Quantification:
1 to 40 ppm (shellfish)

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Shellfish Allergen ELISA kit

Under the Food Allergen Labeling and Consumer Protection Act, crustaceans (shellfish) are among the eight foods or food groups designated as “major food allergens” by congress. Allergies to shellfish occur primarily from the muscle protein tropomyosin in raw and cooked food and often persist throughout adult life, making BioFront’s MonoTrace Crustacean ELISA kit a critical food safety diagnostic tool for the protection of shellfish sensitive individuals.

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Coconut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Shellfish allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference

REACTIVITY

Reacts equally to raw and cooked tropomyosin from shellfish, including shrimp, crab, lobster, prawn, and crayfish.

CROSS-REACTIVITY

The assay exhibits strong reactivity to Crustacea and to a significantly lesser extent showed crossreactivity to octopus (0.0205%). Mussels (0.0013%) demonstrated minimal cross-reactivity when tested with the MonoTrace Crustacea ELISA. At concentrations as high as 100,000 ppm, there was no cross reactivity to tree nuts, legumes, vegetables, seeds, flours or meats. Reactivity towards raw and cooked shrimp, prawn, lobster, crayfish and crab was similar.

RECOVERY

The MonoTrace Crustacea ELISA kit is suitable for the qualitative or quantitative detection of Crustacea within wine, soup mix, fish batter mix, sauces, tomato juice, imitation crab and sesame oil. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In addition, Crustacea was efficiently recovered from shrimp-incurred fish ball samples that were cooked under varying conditions. Commercial samples were evaluated with the MonoTrace Crustacea ELISA. All commercial samples labeled as containing shrimp and/or crab were detected. Lobster was not detected within lobster stock but it was detected within the bisque. In conclusion, raw and cooked Crustacea was efficiently extracted and detected using the MonoTrace Crustacea ELISA from uncooked and cooked food matrices.

Other matrices may also be suitable for the MonoTrace Crustacea ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). An internal validation using a Crustacea-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Shellfish Allergen ELISA kit