Buckwheat Seed Allergen ELISA kitrequest-ifu

Description
Competitive Enzyme immunoassay

Product Code
BW1-EK-96    96 tests
BW1-EK-48    48 tests

Total Assay time
30 minutes

Samples
Buckwheat

Incubation Times:
10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Limit of Detection:
0.08 ppm

Range of Quantification:
1 to 40 ppm

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Buckwheat Seed Allergen ELISA kit

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Buckwheat ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Buckwheat allergen resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

The assay exhibits strong reactivity to buckwheat (Fagopyrum esculentum). At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other seed, nut, legume, or meat matrices tested using the MonoTrace Buckwheat ELISA kit. Minimal cross-reactivity (0.0012-0.0028%) or contamination was observed when certaincommercial grains and spices were assayed.

RECOVERY

The MonoTrace Buckwheat ELISA kit is suitable for the qualitative or quantitative detection of buckwheat within various food matrices including flours, meats, pastas, and cereals. Recoveries were acceptable according to current AOAC guidelines (J. AOAC Int. 2012). In summary, buckwheat was efficiently extracted and detected using the
MonoTrace Buckwheat ELISA from common matrices including flours, meats, pastas, and cereals.

Other matrices may also be suitable for the MonoTrace Buckwheat ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a buckwheat-spiked sample is recommended to ensure that buckwheat is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Buckwheat Seed Allergen ELISA kit