Peanut Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
PA3-EK-96    96 tests
PA3-EK-48    48 tests

Total Assay time
30 minutes


Incubation Times:
10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Limit of Detection:
0.24 ppm

Range of Quantification:
1 to 40 ppm

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Peanut Allergen ELISA kit


BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Peanut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

    – Target a highly stable Peanut allergen resistant to food processing effects
    – Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
    – Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to peanut (Arachis hypogaea). Nutmeg (0.0026%), cayenne pepper (0.0011%), and pumpkin seed (0.0025%) were minimally reactive in the assay. Given the reported adulteration of some commercial spices with peanut in recent years, it is possible that the reactivity observed in certain matrices results from similar circumstances and does not represent cross reactivity within the assay. At concentrations as high as 100,000 ppm, no cross reactivity was observed when testing a variety of other matrices, including tree nuts, legumes, seeds and flours.


The MonoTrace Peanut ELISA kit is suitable for the qualitative or quantitative detection of peanut within
formula, chocolate, confectionary, ice cream and food components such as flours and non-fat dry milk. Confectionaries, chocolate and ice cream extractions require the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is necessary for samples containing polyphenols (which include chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here) and complex polysaccharides (which includes carrageenan and gums such as xanthan, guar, cellulose among others). Recoveries were acceptable, according to AOAC guidelines (J. AOAC Int. 2012). In summary, peanut was efficiently extracted and detected from diverse food matrices.

Other matrices may also be suitable for the MonoTrace Peanut ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a peanut-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

Peanut Allergen ELISA kit