Milk Casein Allergen ELISA kitrequest-ifu

Competitive Enzyme immunoassay

Product Code
CAS-EK-96    96 tests
CAS-EK-48    48 tests

Total Assay time
30 minutes

Bovine casein

Incubation Times:
10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Limit of Detection (bovine casein):
0.12 ppm

Range of Quantification (bovine casein):
1 to 20 ppm

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Milk Casein Allergen ELISA kit

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Milk (Casein) ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target a highly stable Milk allergen (casein) resistant to food processing effects
Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
Perform well in a wide variety of food products with negligible food matrix interference


At concentrations as high as 100,000 ppm, no cross-reactivity was observed in a large panel of assayed samples, including: egg, wheat, almond, cashew, coconut, hazelnut, macadamia nut, peanut, pine nut soybean, lentils, sunflower, mustard, kidney beans, green peas, and lupine.


Recovery of spiked casein samples at various concentrations was efficient (>81%) when the following food matrices were assayed: wheat flour, rice flour, cake mix, and infant soya formula.

Milk Casein Allergen ELISA kit